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human POLK shRNA silencing AAV

Name 
AAV-h-POLK-shRNA
Cat No 
shAAV-219369
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human POLK.

shAAV-219369
AAV-h-POLK-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human POLK (DNA polymerase kappa). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DINB protein; DINB1; DINP; DNA polymerase kappa; POLQ; polymerase (DNA directed) kappa
Description (eCommerce)
DNA polymerase kappa (Pol kappa) is a DNA polymerase specifically involved in DNA repair. It is critical to translesion synthesis and can bypass DNA damage that would typically arrest high-fidelity DNA synthesis.
Description (Vector)
External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 (PubMed 17317631)).(supplied by OMIM, Jan 2010)
Gene ID
51426
Gene Name (eCommerce)
DNA polymerase kappa
Gene Name (Vector)
DNA polymerase kappa
Gene Symbol
POLK
HGNC ID
HGNC:9183
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1419
ORF Size (bp)
1419 bp
Protein Name (eCommerce)
DNA polymerase kappa
RefSeq ID
NM_016218
RefSeq Synonyms
NM_016218, NM_001345922, NM_001345921, BC050718,
Research Areas
Cell Cycle,DNA Damage/Repair,DNA Replication,Fanconi anemia,Replication,Zinc-finger
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
DNA polymerase kappa (Pol kappa) is a DNA polymerase specifically involved in DNA repair. It is critical to translesion synthesis and can bypass DNA damage that would typically arrest high-fidelity DNA synthesis.
UniGene ID
Hs.715657
UniProt ID (eCommerce)
Q9UBT6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.