human PLCE1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human PLCE1.
shAAV-219125
AAV-h-PLCE1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human PLCE1 (phospholipase C epsilon 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
NPHS3; PLCE; PPLC
Description (Vector)
PLCE1 encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID
51196
Gene Name (Vector)
phospholipase C epsilon 1
Gene Symbol
PLCE1
HGNC ID
HGNC:17175
ORF Size (aa)
6909
ORF Size (bp)
6909 bp
RefSeq ID
NM_016341
RefSeq Synonyms
NM_016341, NM_001288989, NM_001165979, BC151854, BC144286, BC140705,
Species
human
UniGene ID
Hs.655033
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.