human PIGV shRNA silencing AAV

Name 
AAV-h-PIGV-shRNA
Cat No 
shAAV-218927
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PIGV.

shAAV-218927
AAV-h-PIGV-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PIGV (phosphatidylinositol glycan anchor biosynthesis class V). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
GPI-MT-II; HPMRS1; PIG-V
Description (Vector)
PIGV encodes a mannosyltransferase enzyme involved in the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a complex glycolipid that functions as a membrane anchor for many proteins and plays a role in multiple cellular processes including protein sorting and signal transduction. The encoded protein is localized to the endoplasmic reticulum and transfers the second mannose to the GPI backbone. Mutations in this gene are associated with hyperphosphatasia mental retardation syndrome. Alternatively spliced transcript variants have been observed for this gene.
Gene ID
55650
Gene Name (Vector)
phosphatidylinositol glycan anchor biosynthesis class V
Gene Symbol
PIGV
HGNC ID
HGNC:26031
ORF Size (aa)
1482
ORF Size (bp)
1482 bp
RefSeq ID
NM_017837
RefSeq Synonyms
NM_017837, NM_001202554, BC013568,
Species
human
UniGene ID
Hs.259605

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.