human PHKB shRNA silencing AAV

Name 
AAV-h-PHKB-shRNA
Cat No 
shAAV-218833
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PHKB.

shAAV-218833
AAV-h-PHKB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PHKB (phosphorylase kinase regulatory subunit beta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
phosphorylase b kinase regulatory subunit beta; phosphorylase kinase beta-subunit; phosphorylase kinase subunit beta; phosphorylase kinase, beta
Description (eCommerce)
Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. Phosphorylase b kinase regulatory subunit beta (PHKB) is the beta chain and acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q93100].
Description (Vector)
Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.
Gene ID
5257
Gene Name (eCommerce)
phosphorylase kinase regulatory subunit beta
Gene Name (Vector)
phosphorylase kinase regulatory subunit beta
Gene Symbol
PHKB
HGNC ID
HGNC:8927
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3261
ORF Size (bp)
3261 bp
Protein Name (eCommerce)
Phosphorylase b kinase regulatory subunit beta
RefSeq ID
NM_000293
RefSeq Synonyms
NM_001031835, NM_000293, BC033657,
Research Areas
Insulin Signaling,Metabolism/Metabolic Process,Signal Transduction
Research Areas (Faceted)
cell_biology,metabolism,signal_transduction
Species
human
Target Sentence
Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. Phosphorylase b kinase regulatory subunit beta (PHKB) is the beta chain and acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q93100].
UniGene ID
Hs.78060
UniProt ID (eCommerce)
Q93100

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.