human PGK2 shRNA silencing AAV

Name
AAV-h-PGK2-shRNA

Cat No
shAAV-218740

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human PGK2.

shAAV-218740

AAV-h-PGK2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PGK2 (phosphoglycerate kinase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

dJ417L20.2; HEL-S-272; PGKB; PGKPS

Description (Vector)

PGK2 is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.

Gene ID

5232

Gene Name (Vector)

phosphoglycerate kinase 2

Gene Symbol

PGK2

HGNC ID

HGNC:8898

ORF Size (aa)

1254

ORF Size (bp)

1254 bp

RefSeq ID

NM_138733

RefSeq Synonyms

NM_138733, BC038843,

Species

human

UniGene ID

Hs.367727

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.