human P4HA3 shRNA silencing AAV

Name
AAV-h-P4HA3-shRNA

Cat No
shAAV-218078

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human P4HA3.

shAAV-218078

AAV-h-P4HA3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human P4HA3 (prolyl 4-hydroxylase subunit alpha 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

P4HA3 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Gene ID

283208

Gene Name (Vector)

prolyl 4-hydroxylase subunit alpha 3

Gene Symbol

P4HA3

HGNC ID

HGNC:30135

ORF Size (aa)

1635

ORF Size (bp)

1635 bp

RefSeq ID

NM_182904

RefSeq Synonyms

NM_182904, NM_001288748, BC126170, BC117333,

Species

human

UniGene ID

Hs.660541

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.