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human NUMB shRNA silencing AAV

Name 
AAV-h-NUMB-shRNA
Cat No 
shAAV-217229
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NUMB.

shAAV-217229
AAV-h-NUMB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NUMB (NUMB, endocytic adaptor protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
74
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
c14_5527; C14orf41; h-Numb; numb homolog; protein numb homolog; Protein S171; S171
Description (eCommerce)
NUMB plays an essential role in the determination of cell fate and development and appears to be especially important to sensory organogenesis and neurogenesis. NUMB is a notch1-interacting protein that antagonizes the notch1 signaling pathway. In the cell, NUMB is asymmetrically localized. The polarized distribution of NUMB in the cell is fundamental to its function of promoting asymmetric cell division for cell fate specification. Numb is also implicated to function as an endocytic protein, as it interacts with EPS15, a protein involved in endocytosis.
Description (Vector)
NUMB plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene.
Gene ID
8650
Gene Name (eCommerce)
NUMB, endocytic adaptor protein
Gene Name (Vector)
NUMB, endocytic adaptor protein
Gene Symbol
NUMB
HGNC ID
HGNC:8060
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1779
ORF Size (bp)
1779 bp
Protein Name (eCommerce)
Protein numb homolog
RefSeq ID
NM_003744
RefSeq Synonyms
NM_003744, NM_001320114, NM_001005745, NM_001005744, NM_001005743, BC068476, BC033824,
Research Areas
Axon Guidance,Cancer,Cardiology,Cardiovascular,HGF Signaling,Neurobiology,Neurodevelopment,Neurogenesis,Notch Signaling,Signal Transduction
Research Areas (Faceted)
cancer,signal_transduction,neurobiology,cardiovascular,signal_transduction,neurobiology,developmental_biology,signal_transduction
Species
human
Target Sentence
NUMB plays an essential role in the determination of cell fate and development and appears to be especially important to sensory organogenesis and neurogenesis. NUMB is a notch1-interacting protein that antagonizes the notch1 signaling pathway. In the cell, NUMB is asymmetrically localized. The polarized distribution of NUMB in the cell is fundamental to its function of promoting asymmetric cell division for cell fate specification. Numb is also implicated to function as an endocytic protein, as it interacts with EPS15, a protein involved in endocytosis.
UniGene ID
Hs.714879
UniProt ID (eCommerce)
P49757

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.