human NUCKS1 shRNA silencing AAV

Name 
AAV-h-NUCKS1-shRNA
Cat No 
shAAV-217183
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NUCKS1.

shAAV-217183
AAV-h-NUCKS1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NUCKS1 (nuclear casein kinase and cyclin dependent kinase substrate 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
JC7; NUCKS; nuclear ubiquitous casein and cyclin-dependent kinase substrate 1; nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate; P1; potential LAG1 interactor
Description (eCommerce)
NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene.
Description (Vector)
NUCKS1 encodes a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated in vivo by Cdk1 during mitosis of the cell cycle.
Gene ID
64710
Gene Name (eCommerce)
nuclear casein kinase and cyclin dependent kinase substrate 1
Gene Name (Vector)
nuclear casein kinase and cyclin dependent kinase substrate 1
Gene Symbol
NUCKS1
HGNC ID
HGNC:29923
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
732
Protein Name (eCommerce)
Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1
RefSeq ID
NM_022731
RefSeq Synonyms
NM_022731, BC000805,
Research Areas
Neurobiology
Research Areas (Faceted)
neurobiology
Species
human
Target Sentence
NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene.
UniGene ID
Hs.652429
UniProt ID (eCommerce)
Q9H1E3

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.