human NPM1 shRNA silencing AAV

Name 
AAV-h-NPM1-shRNA
Cat No 
shAAV-216984
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NPM1.

shAAV-216984
AAV-h-NPM1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NPM1 (nucleophosmin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
B23; NPM; Nucleolar phosphoprotein B23; nucleolar protein NO38; nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin/nucleoplasmin family, member 1; Numatrin; testicular tissue protein Li 128
Description (eCommerce)
Nucleophosmin (NPM1) is involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors TP53/p53 and ARF. It associates with nucleolar ribonucleoprotein structures and binds single-stranded nucleic acids. NPM1 binds ribosomes presumably to drive ribosome nuclear export. Chromosomal aberrations involving NPM1 have been identified in several types of leukemia [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P06748].
Description (Vector)
NPM1 encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
Gene ID
4869
Gene Name (eCommerce)
nucleophosmin 1
Gene Name (Vector)
nucleophosmin
Gene Symbol
NPM1
HGNC ID
HGNC:7910
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
780
Protein Name (eCommerce)
Nucleophosmin
RefSeq ID
NM_002520
RefSeq Synonyms
NM_199185, NM_002520, NM_001037738, BC107754, BC050628, BC021983, BC021668, BC016824, BC016768, BC016716, BC014349, BC012566, BC009623, BC008495, BC002398,
Research Areas
Aging,Apoptosis,Cancer,Cell Cycle,Cytoskeleton,DNA Damage/Repair,Host-Virus Interactions,Nuclear Import/Export,Protein Folding,Replication,RNA Binding,Signal Transduction,Transport
Research Areas (Faceted)
cancer,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation,infectious_disease,cell_biology,cell_cycle,genetics,cell_biology
Species
human
Target Sentence
Nucleophosmin (NPM1) is involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors TP53/p53 and ARF. It associates with nucleolar ribonucleoprotein structures and binds single-stranded nucleic acids. NPM1 binds ribosomes presumably to drive ribosome nuclear export. Chromosomal aberrations involving NPM1 have been identified in several types of leukemia [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P06748].
UniGene ID
Hs.557550
UniProt ID (eCommerce)
P06748

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.