human NPL shRNA silencing AAV

Name 
AAV-h-NPL-shRNA
Cat No 
shAAV-216971
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NPL.

shAAV-216971
AAV-h-NPL-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NPL (N-acetylneuraminate pyruvate lyase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
C112; C1orf13; NAL; NPL1
Description (Vector)
NPL encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Gene ID
80896
Gene Name (Vector)
N-acetylneuraminate pyruvate lyase
Gene Symbol
NPL
HGNC ID
HGNC:16781
ORF Size (aa)
963
ORF Size (bp)
963 bp
RefSeq ID
NM_030769
RefSeq Synonyms
NM_030769, NM_001200056, NM_001200052, NM_001200051, NM_001200050, BC125051, BC058003,
Species
human
UniGene ID
Hs.496969

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.