human NPHS1 shRNA silencing AAV

Name
AAV-h-NPHS1-shRNA

Cat No
shAAV-216962

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human NPHS1.

shAAV-216962

AAV-h-NPHS1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NPHS1 (NPHS1, nephrin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CNF; nephrin; NPHN

Description (Vector)

NPHS1 encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Gene ID

4868

Gene Name (Vector)

NPHS1, nephrin

Gene Symbol

NPHS1

HGNC ID

HGNC:7908

ORF Size (aa)

3726

ORF Size (bp)

3726 bp

RefSeq ID

NM_004646

RefSeq Synonyms

NM_004646, BC156935, BC156195,

Species

human

UniGene ID

Hs.122186

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.