human NOS3 shRNA silencing AAV

Name 
AAV-h-NOS3-shRNA
Cat No 
shAAV-216906
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NOS3.

shAAV-216906
AAV-h-NOS3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NOS3 (nitric oxide synthase 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
cNOS; constitutive NOS; ECNOS; EC-NOS; endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase, endothelial; NOS type III; NOSIII
Description (eCommerce)
endothelial nitric oxide synthase (eNOS) is one of four major isoforms of nitric oxide synthase (NOS). The four isoforms, neuronal NOS, endothelial NOS, inducible NOS, and mitochondrial NOS, catalyze the synthesis of nitric oxide (NO) from L-arginine and oxygen. NO is a small free radical that functions as a key signaling molecule in the cardiovascular and neural systems. eNOS is responsible for most of the NO production in the vascular system where it mediates smooth muscle relaxation, angiogenesis, and clotting.
Description (Vector)
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
4846
Gene Name (eCommerce)
nitric oxide synthase 3
Gene Name (Vector)
nitric oxide synthase 3
Gene Symbol
NOS3
HGNC ID
HGNC:7876
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3612
ORF Size (bp)
3612 bp
Protein Name (eCommerce)
Nitric oxide synthase, endothelial
RefSeq ID
NM_000603
RefSeq Synonyms
NM_001160111, NM_001160110, NM_001160109, NM_000603, BC069465, BC063294,
Research Areas
Aging,Angiogenesis,Apoptosis,Cancer,Cardiology,Cardiovascular,Cytoskeleton,EGFR Signaling,Estrogen Signaling,Golgi Apparatus,HIF-1 Signaling,Hypoxia,Leptin Signaling,Metabolism/Metabolic Process,PI3K/Akt Signaling,Signal Transduction,VEGF Signaling
Research Areas (Faceted)
cancer,signal_transduction,cell_biology,developmental_biology,cell_biology,cardiovascular,signal_transduction,cell_biology,metabolism,signal_transduction,signal_transduction
Species
human
Target Sentence
endothelial nitric oxide synthase (eNOS) is one of four major isoforms of nitric oxide synthase (NOS). The four isoforms, neuronal NOS, endothelial NOS, inducible NOS, and mitochondrial NOS, catalyze the synthesis of nitric oxide (NO) from L-arginine and oxygen. NO is a small free radical that functions as a key signaling molecule in the cardiovascular and neural systems. eNOS is responsible for most of the NO production in the vascular system where it mediates smooth muscle relaxation, angiogenesis, and clotting.
UniGene ID
Hs.647092
UniProt ID (eCommerce)
P29474

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.