human NMT1 shRNA silencing AAV

Name
AAV-h-NMT1-shRNA

Cat No
shAAV-216836

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human NMT1.

shAAV-216836

AAV-h-NMT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NMT1 (N-myristoyltransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

alternative, short form NMT-S; glycylpeptide N-tetradecanoyltransferase 1; long form, NMT-L; myristoyl-CoA:protein N-myristoyltransferase 1; NMT; NMT 1; Peptide N-myristoyltransferase 1; type I N-myristoyltransferase

Description (eCommerce)

NMT1 adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P30419].

Description (Vector)

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 (PubMed 1570339)).(supplied by OMIM, Nov 2008)

Gene ID

4836

Gene Name (eCommerce)

N-myristoyltransferase 1

Gene Name (Vector)

N-myristoyltransferase 1

Gene Symbol

NMT1

HGNC ID

HGNC:7857

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1491

ORF Size (bp)

1491 bp

Protein Name (eCommerce)

Glycylpeptide N-tetradecanoyltransferase 1

RefSeq ID

NM_021079

RefSeq Synonyms

NM_021079, BC006569, BC006538,

Research Areas

Acyltransferase,Apoptosis,Cytoskeleton,Host-Virus Interactions,Neurobiology,Signal Transduction

Research Areas (Faceted)

signal_transduction,cell_biology,infectious_disease,neurobiology

Species

human

Target Sentence

NMT1 adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P30419].

UniGene ID

Hs.532790

UniProt ID (eCommerce)

P30419

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.