human NFU1 shRNA silencing AAV

Name
AAV-h-NFU1-shRNA

Cat No
shAAV-216660

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human NFU1.

shAAV-216660

AAV-h-NFU1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NFU1 (NFU1 iron-sulfur cluster scaffold). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CGI-33; HIRIP; HIRIP5; MMDS1; Nfu; NifU; NIFUC

Description (Vector)

NFU1 encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene ID

27247

Gene Name (Vector)

NFU1 iron-sulfur cluster scaffold

Gene Symbol

NFU1

HGNC ID

HGNC:16287

ORF Size (aa)

765

RefSeq ID

NM_015700

RefSeq Synonyms

NM_015700, NM_001002757, NM_001002756, NM_001002755, BC113694, BC113692,

Species

human

UniGene ID

Hs.430439

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.