human NFIC shRNA silencing AAV

Name 
AAV-h-NFIC-shRNA
Cat No 
shAAV-216641
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NFIC.

shAAV-216641
AAV-h-NFIC-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NFIC (nuclear factor I C). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CCAAT-box-binding transcription factor; CTF; CTF5; NF1-C; NFI; NF-I; NF-I/C; NFI-C; nuclear factor 1 C-type; Nuclear factor 1/C; Nuclear factor I/C; nuclear factor I/C (CCAAT-binding transcription factor); TGGCA-binding protein
Description (eCommerce)
Nuclear factor I/C (NFIC) recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08651].
Description (Vector)
NFIC belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Gene ID
4782
Gene Name (eCommerce)
nuclear factor I C
Gene Name (Vector)
nuclear factor I C
Gene Symbol
NFIC
HGNC ID
HGNC:7786
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1287
ORF Size (bp)
1287 bp
Protein Name (eCommerce)
Nuclear factor 1 C-type
RefSeq ID
NM_005597
RefSeq Synonyms
NM_205843, NM_005597, NM_001245005, NM_001245004, NM_001245002, BC012120,
Research Areas
DNA Replication,Gene Expression,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,genetics,transcription_translation
Species
human
Target Sentence
Nuclear factor I/C (NFIC) recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08651].
UniGene ID
Hs.170131
UniProt ID (eCommerce)
P08651

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.