human NEK3 shRNA silencing AAV

Name
AAV-h-NEK3-shRNA

Cat No
shAAV-216571

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human NEK3.

shAAV-216571

AAV-h-NEK3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NEK3 (NIMA related kinase 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

HSPK36

Description (Vector)

NEK3 encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene ID

4752

Gene Name (Vector)

NIMA related kinase 3

Gene Symbol

NEK3

HGNC ID

HGNC:7746

ORF Size (aa)

1521

ORF Size (bp)

1521 bp

RefSeq ID

NM_152720

RefSeq Synonyms

NM_152720, NM_002498, NM_001146099, BC019916,

Species

human

UniGene ID

Hs.409989

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.