human NEDD4L shRNA silencing AAV

Name
AAV-h-NEDD4L-shRNA

Cat No
shAAV-216553

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human NEDD4L.

shAAV-216553

AAV-h-NEDD4L-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NEDD4L (neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

E3 ubiquitin-protein ligase NEDD4-like; HECT-type E3 ubiquitin transferase NED4L; hNEDD4-2; NEDD4.2; NEDD4-2; PVNH7; RSP5; ubiquitin-protein ligase Rsp5

Description (eCommerce)

NEDD4L (neural precursor cell expressed, developmentally down-regulated 4-like) functions as an E3 ubiquitin-protein ligase. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. It also promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5 [taken from the Universal Protein Resource (UniProt) wwwuniprotorg/uniprot/Q96PU5].

Description (Vector)

NEDD4L encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene ID

23327

Gene Name (eCommerce)

neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase

Gene Name (Vector)

neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase

Gene Symbol

NEDD4L

HGNC ID

HGNC:7728

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2736

ORF Size (bp)

2736 bp

Protein Name (eCommerce)

E3 ubiquitin-protein ligase NEDD4-like

RefSeq ID

NM_015277

RefSeq Synonyms

NM_015277, NM_001243960, NM_001144971, NM_001144970, NM_001144969, NM_001144968, NM_001144967, NM_001144966, NM_001144965, NM_001144964, BC032597,

Research Areas

Antigen Processing and Presentation,Gene Expression,Host-Virus Interactions,Immunology,Neurobiology,Signal Transduction,TGF-beta Signaling,Transcription Factor/Regulator,Ubiquitination

Research Areas (Faceted)

cell_biology,immunology,infectious_disease,neurobiology,signal_transduction,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.602641

UniProt ID (eCommerce)

Q96PU5

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.