human NDUFB5 shRNA silencing AAV

Name 
AAV-h-NDUFB5-shRNA
Cat No 
shAAV-216509
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NDUFB5.

shAAV-216509
AAV-h-NDUFB5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NDUFB5 (NADH:ubiquinone oxidoreductase subunit B5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CISGDH; SGDH
Description (Vector)
NDUFB5 is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene.
Gene ID
4711
Gene Name (Vector)
NADH:ubiquinone oxidoreductase subunit B5
Gene Symbol
NDUFB5
HGNC ID
HGNC:7700
ORF Size (aa)
414
RefSeq ID
NM_002492
RefSeq Synonyms
NM_002492, NM_001199958, NM_001199957, BC093071, BC009796, BC005271,
Species
human
UniGene ID
Hs.718447

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.