human NCOA5 shRNA silencing AAV

Name 
AAV-h-NCOA5-shRNA
Cat No 
shAAV-216423
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NCOA5.

shAAV-216423
AAV-h-NCOA5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NCOA5 (nuclear receptor coactivator 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
bA465L10.6; CIA; coactivator independent of AF-2; NCoA-5; nuclear receptor coactivator 5
Description (eCommerce)
NCOA5 (Nuclear Receptor Coactivator 5), also known as CIA (Coactivator Independent of AF-2 function), has been shown interact exclusively with estrogen receptor alpha (ERalpha) and beta (ERbeta) to function as a coactivator and enhance transcriptional activation by ER. NCOA5 has also been demonstrated to function as a corepressor of MYC/c-myc in response to 17-beta estradiol.
Description (Vector)
NCOA5 encodes a coregulator for the alpha and beta estrogen receptors and the orphan nuclear receptor NR1D2. The protein localizes to the nucleus, and is thought to have both coactivator and corepressor functions. Its interaction with nuclear receptors is independent of the AF2 domain on the receptors, which is known to regulate interaction with other coreceptors. Two alternatively spliced transcript variants for this gene have been described. However, the full length nature of one of the variants has not been determined.
Gene ID
57727
Gene Name (eCommerce)
nuclear receptor coactivator 5
Gene Name (Vector)
nuclear receptor coactivator 5
Gene Symbol
NCOA5
HGNC ID
HGNC:15909
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1740
ORF Size (bp)
1740 bp
Protein Name (eCommerce)
Nuclear receptor coactivator 5
RefSeq ID
NM_020967
RefSeq Synonyms
NM_020967, BC151133, BC140836, BC056872,
Research Areas
Nuclear Receptor Coactivators,Transcription Factor/Regulator,Translational Control
Research Areas (Faceted)
cell_biology,transcription_translation
Species
human
Target Sentence
NCOA5 (Nuclear Receptor Coactivator 5), also known as CIA (Coactivator Independent of AF-2 function), has been shown interact exclusively with estrogen receptor alpha (ERalpha) and beta (ERbeta) to function as a coactivator and enhance transcriptional activation by ER. NCOA5 has also been demonstrated to function as a corepressor of MYC/c-myc in response to 17-beta estradiol.
UniGene ID
Hs.654991
UniProt ID (eCommerce)
Q9HCD5

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.