human NAGPA shRNA silencing AAV

Name
AAV-h-NAGPA-shRNA

Cat No
shAAV-216292

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human NAGPA.

shAAV-216292

AAV-h-NAGPA-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NAGPA (N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

APAA; UCE

Description (Vector)

Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. This reaction most likely occurs in the trans-Golgi network. This enzyme functions as a homotetramer of two disulfide-linked homodimers. In addition to having an N-terminal signal peptide, the protein's C-terminus contains multiple signals for trafficking it between lysosomes, the plasma membrane, and trans-Golgi network.

Gene ID

51172

Gene Name (Vector)

N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Gene Symbol

NAGPA

HGNC ID

HGNC:17378

ORF Size (aa)

930

ORF Size (bp)

930 bp

RefSeq ID

NM_016256

RefSeq Synonyms

NM_016256, BC012194,

Species

human

UniGene ID

Hs.21334

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.