human MRPL50 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human MRPL50.
shAAV-215834
AAV-h-MRPL50-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human MRPL50 (mitochondrial ribosomal protein L50). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
MRP-L50
Description (Vector)
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.
Gene ID
54534
Gene Name (Vector)
mitochondrial ribosomal protein L50
Gene Symbol
MRPL50
HGNC ID
HGNC:16654
ORF Size (aa)
477
RefSeq ID
NM_019051
RefSeq Synonyms
NM_019051, BC032008,
Species
human
UniGene ID
Hs.288224
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.