human MRPL47 shRNA silencing AAV

Name
AAV-h-MRPL47-shRNA

Cat No
shAAV-215831

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MRPL47.

shAAV-215831

AAV-h-MRPL47-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MRPL47 (mitochondrial ribosomal protein L47). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CGI-204; L47mt; MRP-L47; NCM1

Description (Vector)

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified.

Gene ID

57129

Gene Name (Vector)

mitochondrial ribosomal protein L47

Gene Symbol

MRPL47

HGNC ID

HGNC:16652

ORF Size (aa)

693

RefSeq ID

NM_020409

RefSeq Synonyms

NM_177988, NM_020409, BC032522, BC021575,

Species

human

UniGene ID

Hs.283734

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.