human MRPL18 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human MRPL18.
shAAV-215788
AAV-h-MRPL18-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human MRPL18 (mitochondrial ribosomal protein L18). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
HSPC071; L18mt; MRP-L18
Description (Vector)
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L18P ribosomal protein family. Three polymorphic sites exist in this gene, one of which is three nt in length which causes an extra aa near the N-terminus.
Gene ID
29074
Gene Name (Vector)
mitochondrial ribosomal protein L18
Gene Symbol
MRPL18
HGNC ID
HGNC:14477
ORF Size (aa)
543
RefSeq ID
NM_014161
RefSeq Synonyms
NM_014161, NM_001318817, BC001623,
Species
human
UniGene ID
Hs.416998
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.