human MRPL10 shRNA silencing AAV

Name
AAV-h-MRPL10-shRNA

Cat No
shAAV-215775

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MRPL10.

shAAV-215775

AAV-h-MRPL10-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MRPL10 (mitochondrial ribosomal protein L10). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

L10MT; MRP-L10; MRP-L8; MRPL8; RPML8

Description (Vector)

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q.

Gene ID

124995

Gene Name (Vector)

mitochondrial ribosomal protein L10

Gene Symbol

MRPL10

HGNC ID

HGNC:14055

ORF Size (aa)

786

RefSeq ID

NM_148887

RefSeq Synonyms

NM_148887, NM_145255, BC052601, BC015904,

Species

human

UniGene ID

Hs.347535

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.