human MGEA5 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human MGEA5.
shAAV-215423
AAV-h-MGEA5-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human MGEA5 (meningioma expressed antigen 5 (hyaluronidase)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
beta-hexosaminidase; beta-N-acetylglucosaminidase; beta-N-acetylhexosaminidase; bifunctional protein NCOAT; hyaluronidase in meningioma; MEA5; meningioma expressed antigen 5 (hyaluronidase); meningioma-expressed antigen 5; MGEA5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; OGA; protein O-GlcNAcase
Description (eCommerce)
Meningioma-expressed antigen 5 (MGEA5) cleaves GlcNAc but not GalNAc from glycopeptides. MGEA5 can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc.MGEA5 possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3Â [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60502].
Description (Vector)
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 (PubMed 11148210)).(supplied by OMIM, Mar 2008)
Gene ID
10724
Gene Name (eCommerce)
O-GlcNAcase
Gene Name (Vector)
meningioma expressed antigen 5 (hyaluronidase)
Gene Symbol
MGEA5
HGNC ID
HGNC:7056
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2550
ORF Size (bp)
2550 bp
Protein Name (eCommerce)
Protein O-GlcNAcase
RefSeq ID
NM_012215
RefSeq Synonyms
NM_012215, NM_001142434, BC039583,
Research Areas
Acyltransferase,Aging,Apoptosis,Cardiology,Cell Biology,Insulin Signaling,Metabolism/Metabolic Process,Mitochondrion,Transport
Research Areas (Faceted)
cardiovascular,cell_biology,metabolism,signal_transduction
Species
human
Target Sentence
Meningioma-expressed antigen 5 (MGEA5) cleaves GlcNAc but not GalNAc from glycopeptides. MGEA5 can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc.MGEA5 possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3Â [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60502].
UniGene ID
Hs.500842
UniProt ID (eCommerce)
O60502
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.