human METAP2 shRNA silencing AAV

Name 
AAV-h-METAP2-shRNA
Cat No 
shAAV-215321
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human METAP2.

shAAV-215321
AAV-h-METAP2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human METAP2 (methionyl aminopeptidase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MetAP 2; methionine aminopeptidase 2; MNPEP; p67; p67eIF2; Peptidase M; peptidase M 2; testicular tissue protein Li 17
Description (eCommerce)
Methionine aminopeptidase 2 (MetAP) removes the amino-terminal methionine from nascent proteins. The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P50579].
Description (Vector)
METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2.
Gene ID
10988
Gene Name (eCommerce)
methionyl aminopeptidase 2
Gene Name (Vector)
methionyl aminopeptidase 2
Gene Symbol
METAP2
HGNC ID
HGNC:16672
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1437
ORF Size (bp)
1437 bp
Protein Name (eCommerce)
Methionine aminopeptidase 2
RefSeq ID
NM_006838
RefSeq Synonyms
NM_006838, NM_001330246, NM_001317183, NM_001317182, BC013782,
Research Areas
Neurobiology,Signal Transduction
Research Areas (Faceted)
signal_transduction,neurobiology
Species
human
Target Sentence
Methionine aminopeptidase 2 (MetAP) removes the amino-terminal methionine from nascent proteins. The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P50579].
UniGene ID
Hs.444986
UniProt ID (eCommerce)
P50579

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.