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human MED16 shRNA silencing AAV

Name 
AAV-h-MED16-shRNA
Cat No 
shAAV-215244
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human MED16.

shAAV-215244
AAV-h-MED16-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MED16 (mediator complex subunit 16). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DRIP92; Mediator complex subunit 16; mediator of RNA polymerase II transcription subunit 16; THRAP5; thyroid hormone receptor-associated protein 5; thyroid hormone receptor-associated protein complex 95 kDa component; thyroid hormone receptor-associated protein, 95-kD subunit; TRAP95; vitamin D3 receptor-interacting protein complex 92 kDa component
Description (eCommerce)
Mediator of RNA polymerase II transcription subunit 16 (MED16) is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y2X0].
Gene ID
10025
Gene Name (eCommerce)
mediator complex subunit 16
Gene Name (Vector)
mediator complex subunit 16
Gene Symbol
MED16
HGNC ID
HGNC:17556
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2526
ORF Size (bp)
2526 bp
Protein Name (eCommerce)
Mediator of RNA polymerase II transcription subunit 16
RefSeq ID
NM_005481
RefSeq Synonyms
NM_005481, BC017282, BC011841, BC004554,
Research Areas
Gene Expression,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,transcription_translation
Species
human
Target Sentence
Mediator of RNA polymerase II transcription subunit 16 (MED16) is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y2X0].
UniGene ID
Hs.365207
UniProt ID (eCommerce)
Q9Y2X0

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.