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human MDC1 shRNA silencing AAV

Name 
AAV-h-MDC1-shRNA
Cat No 
shAAV-215208
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human MDC1.

shAAV-215208
AAV-h-MDC1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MDC1 (mediator of DNA damage checkpoint 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
homologue to Drosophila photoreceptor protein calphotin; mediator of DNA damage checkpoint protein 1; NFBD1; nuclear factor with BRCT domains 1
Description (eCommerce)
Mediator of DNA damage checkpoint protein 1 (Mdc1) is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. It is also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5PSV9].
Description (Vector)
MDC1 contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
Gene ID
9656
Gene Name (eCommerce)
mediator of DNA damage checkpoint 1
Gene Name (Vector)
mediator of DNA damage checkpoint 1
Gene Symbol
MDC1
HGNC ID
HGNC:21163
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
5409
ORF Size (bp)
5409 bp
Protein Name (eCommerce)
Mediator of DNA damage checkpoint protein 1
RefSeq ID
NM_014641
RefSeq Synonyms
NM_014641, BC152556,
Research Areas
Cell Cycle,DNA Damage/Repair
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
Mediator of DNA damage checkpoint protein 1 (Mdc1) is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. It is also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5PSV9].
UniGene ID
Hs.653495
UniProt ID (eCommerce)
Q14676

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.