human MCM7 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human MCM7.
shAAV-215190
AAV-h-MCM7-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human MCM7 (minichromosome maintenance complex component 7). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
CDC47; CDC47 homolog; DNA replication licensing factor MCM7; homolog of S. cerevisiae Cdc47; MCM2; minichromosome maintenance deficient 7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104; protein phosphatase 1, regulatory subunit 104
Description (eCommerce)
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM7 is part of the core MCM complex that includes MCM4, MCM6, and MCM7. The core complex possesses DNA helicase activity. MCM7 has been shown to interact with CDK4 and p27Kip1 to regulate cell cycle progression. MCM7 has also been shown to interact with ATR-interacting protein (ATRIP)-ATR to function as a transmitter of DNA damage signals from replication forks to S-phase checkpoint machinery.
Description (Vector)
MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Gene ID
4176
Gene Name (eCommerce)
minichromosome maintenance complex component 7
Gene Name (Vector)
minichromosome maintenance complex component 7
Gene Symbol
MCM7
HGNC ID
HGNC:6950
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1170
ORF Size (bp)
1170 bp
Protein Name (eCommerce)
DNA replication licensing factor MCM7
RefSeq ID
NM_005916
RefSeq Synonyms
NM_182776, NM_005916, NM_001278595, BC013375, BC009398,
Research Areas
Cancer,Cell Cycle,Chromatin Remodeling,DNA Damage/Repair,DNA Replication,Helicase Activity,Kinase/Phosphatase,Replication,Signal Transduction,TNF Signaling,Transcription Factor/Regulator
Research Areas (Faceted)
cancer,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM7 is part of the core MCM complex that includes MCM4, MCM6, and MCM7. The core complex possesses DNA helicase activity. MCM7 has been shown to interact with CDK4 and p27Kip1 to regulate cell cycle progression. MCM7 has also been shown to interact with ATR-interacting protein (ATRIP)-ATR to function as a transmitter of DNA damage signals from replication forks to S-phase checkpoint machinery.
UniGene ID
Hs.438720
UniProt ID (eCommerce)
P33993
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.