human MAP4K4 shRNA silencing AAV

Name
AAV-h-MAP4K4-shRNA

Cat No
shAAV-214953

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MAP4K4.

shAAV-214953

AAV-h-MAP4K4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MAP4K4 (mitogen-activated protein kinase kinase kinase kinase 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

epididymis secretory protein Li 31; FLH21957; HEL-S-31; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; mitogen-activated protein kinase kinase kinase kinase 4; nck-interacting kinase; NIK; Ste20 group protein kinase HGK

Description (eCommerce)

MAP4K4 is a serine/threonine kinase that signals upstream of the c-Jun N-terminal kinase (JNK) and is involved in cell motility. MAP4K4 appears to partially mediate TNF alpha signaling. In skeletal muscle TNF-alpha induces insulin resistance. TNF-alpha induced insulin resistance has been shown to be dependent on MAPK4K4 activity. Interestingly, TNF-alpha regulates MAP4K4 expression and can stimulate MAP4K4 gene expression via TNFR2 (TNFalpha receptor 2) and the c-Jun and ATF2 (activating transcription factor 2) transcription factors. MAP4K4 is highly expressed in most tumor cell lines, and the analysis of MAP4K4 mutants indicates that it may have an important role in cell transformation and invasiveness.

Description (Vector)

MAP4K4 is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.

Gene ID

9448

Gene Name (eCommerce)

mitogen-activated protein kinase kinase kinase kinase 4

Gene Name (Vector)

mitogen-activated protein kinase kinase kinase kinase 4

Gene Symbol

MAP4K4

HGNC ID

HGNC:6866

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

3960

ORF Size (bp)

3960 bp

Protein Name (eCommerce)

Mitogen-activated protein kinase kinase kinase kinase 4

RefSeq ID

NM_145687

RefSeq Synonyms

NM_145687, NM_145686, NM_004834, NM_001242560, NM_001242559, BC156887, BC156089,

Research Areas

CD40 Signaling,Gene Expression,Host-Virus Interactions,Immunology,Insulin Signaling,Interleukin Signaling,JNK Signaling,Kinase/Phosphatase,MAPK Signaling,NF-kB Signaling,Signal Transduction,TNF Signaling

Research Areas (Faceted)

cell_biology,immunology,infectious_disease,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.431550

UniProt ID (eCommerce)

O95819

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.