human MAP3K3 shRNA silencing AAV

Name 
AAV-h-MAP3K3-shRNA
Cat No 
shAAV-214930
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human MAP3K3.

shAAV-214930
AAV-h-MAP3K3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MAP3K3 (mitogen-activated protein kinase kinase kinase 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
MAP/ERK kinase kinase 3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; mitogen-activated protein kinase kinase kinase 3
Description (eCommerce)
MEK (MAPK/ERK kinase) kinase 3 (MEKK3) is a component of a protein kinase signal transduction cascade. MEKK3 has been shown to mediate activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q99759].
Description (Vector)
MAP3K3 product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene ID
4215
Gene Name (eCommerce)
mitogen-activated protein kinase kinase kinase 3
Gene Name (Vector)
mitogen-activated protein kinase kinase kinase 3
Gene Symbol
MAP3K3
HGNC ID
HGNC:6855
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1881
ORF Size (bp)
1881 bp
Protein Name (eCommerce)
Mitogen-activated protein kinase kinase kinase 3
RefSeq ID
NM_002401
RefSeq Synonyms
NM_203351, NM_002401, NM_001330431, BC093674, BC093672, BC090859, BC010464,
Research Areas
Cytokine Signaling,EGFR Signaling,EPO Signaling,GnRH Signaling,Host-Virus Interactions,Immunology,Insulin Signaling,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,MAPK Signaling,Neurobiology,NF-kB Signaling,Signal Transduction,TNF Signaling,VEGF Signaling
Research Areas (Faceted)
cell_biology,immunology,infectious_disease,neurobiology,signal_transduction
Species
human
Target Sentence
MEK (MAPK/ERK kinase) kinase 3 (MEKK3) is a component of a protein kinase signal transduction cascade. MEKK3 has been shown to mediate activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q99759].
UniGene ID
Hs.29282
UniProt ID (eCommerce)
Q99759

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.