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human LIMA1 shRNA silencing AAV

Name 
AAV-h-LIMA1-shRNA
Cat No 
shAAV-213943
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human LIMA1.

shAAV-213943
AAV-h-LIMA1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human LIMA1 (LIM domain and actin binding 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1110021C24Rik; Epithelial protein lost in neoplasm; epithelial protein lost in neoplasm beta; EPLIN; LDLCQ8; LIM domain and actin-binding protein 1; SREBP3; sterol regulatory element binding protein 3
Description (eCommerce)
Epithelial protein lost in neoplasm (EPLIN) is a cytoskeleton-associated protein found to be down-regulated in transformed prostate and breast cells. EPLIN is involved in actin dynamics and plays a role in the formation of stable actin filament structures.
Description (Vector)
LIMA1 encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated.
Gene ID
51474
Gene Name (eCommerce)
LIM domain and actin binding 1
Gene Name (Vector)
LIM domain and actin binding 1
Gene Symbol
LIMA1
HGNC ID
HGNC:24636
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2280
ORF Size (bp)
2280 bp
Protein Name (eCommerce)
LIM domain and actin-binding protein 1
RefSeq ID
NM_016357
RefSeq Synonyms
NM_016357, NM_001243775, NM_001113547, NM_001113546, BC136763, BC110815,
Research Areas
Cancer,Cytoskeleton,Tumor Suppressors/Oncoproteins
Research Areas (Faceted)
cancer,cell_biology
Species
human
Target Sentence
Epithelial protein lost in neoplasm (EPLIN) is a cytoskeleton-associated protein found to be down-regulated in transformed prostate and breast cells. EPLIN is involved in actin dynamics and plays a role in the formation of stable actin filament structures.
UniGene ID
Hs.525419
UniProt ID (eCommerce)
Q9UHB6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.