human LIG4 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human LIG4.
shAAV-213923
AAV-h-LIG4-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human LIG4 (DNA ligase 4). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
LIG4S
Description (Vector)
LIG4 is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Gene ID
3981
Gene Name (Vector)
DNA ligase 4
Gene Symbol
LIG4
HGNC ID
HGNC:6601
ORF Size (aa)
2736
ORF Size (bp)
2736 bp
RefSeq ID
NM_002312
RefSeq Synonyms
NM_206937, NM_002312, NM_001330595, NM_001098268, BC037491,
Species
human
UniGene ID
Hs.166091
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.