human ITGAE shRNA silencing AAV

Name 
AAV-h-ITGAE-shRNA
Cat No 
shAAV-212489
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ITGAE.

shAAV-212489
AAV-h-ITGAE-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ITGAE (integrin subunit alpha E). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
50
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
alpha polypeptide; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD antigen CD103; CD103; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; HUMINAE; integrin alpha-E; integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1); mucosal lymphocyte 1 antigen
Description (eCommerce)
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD103/ITGAE is an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103/ITGAE forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen, and is preferentially expressed in human intestinal intraepithelial T-lymphocytes (IEL). In addition to a role in adhesion, CD103/ITGAE may serve as an accessory molecule for IEL activation. [taken from NCBI Entrez Gene (Gene ID: 3682)].
Description (Vector)
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation.
Gene ID
3682
Gene Name (eCommerce)
integrin subunit alpha E
Gene Name (Vector)
integrin subunit alpha E
Gene Symbol
ITGAE
HGNC ID
HGNC:6147
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3540
ORF Size (bp)
3540 bp
Protein Name (eCommerce)
Integrin alpha-E
RefSeq ID
NM_002208
RefSeq Synonyms
NM_002208, BC117207, BC113436,
Research Areas
Cytoskeleton,Signal Transduction
Research Areas (Faceted)
cell_biology,signal_transduction
Species
human
Target Sentence
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD103/ITGAE is an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103/ITGAE forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen, and is preferentially expressed in human intestinal intraepithelial T-lymphocytes (IEL). In addition to a role in adhesion, CD103/ITGAE may serve as an accessory molecule for IEL activation. [taken from NCBI Entrez Gene (Gene ID: 3682)].
UniGene ID
Hs.513867
UniProt ID (eCommerce)
P38570

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.