human ITCH shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human ITCH.
shAAV-212458
AAV-h-ITCH-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human ITCH (itchy E3 ubiquitin protein ligase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
ADMFD; AIF4; AIP4; atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; E3 ubiquitin-protein ligase Itchy homolog; HECT-type E3 ubiquitin transferase Itchy homolog; Itch; itchy E3 ubiquitin protein ligase homolog; NAPP1; NFE2-associated polypeptide 1
Description (eCommerce)
Itch is an HECT-type E3 ubiquitin ligase that promotes the ubiquitination of Notch. The drosophila ortholog of Itch, suppressor of deltex (SU(DX)), was originally identified as a negative regulator of the Notch pathway. Deltex is a transmembrane ligand for Notch and also has a mammalian homolog, DTX1.The human ortholog of Itch and (SU(DX)) is called AIP4 (atrophin-1-interacting protein) and was first described as a protein that interacts with the product of the gene responsible for dentatorubral and pallidoluysian atrophy (DRPLA). In mice, Itch was identified as a mutant locus in 18H mice that resulted in a fatal disease characterized by inflammation of the skin which resulted in constant itching and scarring.
Description (Vector)
ITCH encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Gene ID
83737
Gene Name (eCommerce)
itchy E3 ubiquitin protein ligase
Gene Name (Vector)
itchy E3 ubiquitin protein ligase
Gene Symbol
ITCH
HGNC ID
HGNC:13890
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2712
ORF Size (bp)
2712 bp
Protein Name (eCommerce)
E3 ubiquitin-protein ligase Itchy homolog
RefSeq ID
NM_031483
RefSeq Synonyms
NM_031483, NM_001324198, NM_001324197, NM_001257138, NM_001257137, BC011571,
Research Areas
Antigen Processing and Presentation,Apoptosis,Cancer,EGFR Signaling,Host-Virus Interactions,IFN Signaling,Immunology,Innate Immunity,JNK Signaling,Notch Signaling,Response to Virus,Signal Transduction,TNF Signaling,Ubiquitination
Research Areas (Faceted)
cancer,cell_biology,developmental_biology,immunology,infectious_disease,signal_transduction
Species
human
Target Sentence
Itch is an HECT-type E3 ubiquitin ligase that promotes the ubiquitination of Notch. The drosophila ortholog of Itch, suppressor of deltex (SU(DX)), was originally identified as a negative regulator of the Notch pathway. Deltex is a transmembrane ligand for Notch and also has a mammalian homolog, DTX1.The human ortholog of Itch and (SU(DX)) is called AIP4 (atrophin-1-interacting protein) and was first described as a protein that interacts with the product of the gene responsible for dentatorubral and pallidoluysian atrophy (DRPLA). In mice, Itch was identified as a mutant locus in 18H mice that resulted in a fatal disease characterized by inflammation of the skin which resulted in constant itching and scarring.
UniGene ID
Hs.632272
UniProt ID (eCommerce)
Q96J02
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.