human INSR shRNA silencing AAV

Name 
AAV-h-INSR-shRNA
Cat No 
shAAV-212314
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human INSR.

shAAV-212314
AAV-h-INSR-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human INSR (insulin receptor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CD antigen CD220; CD220; HHF5; insulin receptor; IR
Description (eCommerce)
The insulin receptor is a receptor tyrosine kinase that mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates to stimulate uptake of glucose. The insulin receptor is synthesized as a single polypeptide that is cleaved to generate an alpha and beta subunit. The subunit structure of the insulin receptor consists of a tetramer of two alpha and two beta chains linked by disulfide bonds.
Description (Vector)
After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
3643
Gene Name (eCommerce)
insulin receptor
Gene Name (Vector)
insulin receptor
Gene Symbol
INSR
HGNC ID
HGNC:6091
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4113
ORF Size (bp)
4113 bp
Protein Name (eCommerce)
Insulin receptor
RefSeq ID
NM_000208
RefSeq Synonyms
NM_001079817, NM_000208, BC117172,
Research Areas
Cancer,Cardiac Development,Cardiology,Cardiovascular,Cell Cycle,DNA Damage/Repair,DNA Replication,EGFR Signaling,EPO Signaling,Gene Expression,HIF-1 Signaling,Host-Virus Interactions,Insulin Signaling,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,MAPK Signaling,Metabolism/Metabolic Process,Neurobiology,PI3K/Akt Signaling,Signal Transduction,Synapse,VEGF Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,immunology,infectious_disease,metabolism,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
The insulin receptor is a receptor tyrosine kinase that mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates to stimulate uptake of glucose. The insulin receptor is synthesized as a single polypeptide that is cleaved to generate an alpha and beta subunit. The subunit structure of the insulin receptor consists of a tetramer of two alpha and two beta chains linked by disulfide bonds.
UniGene ID
Hs.465744
UniProt ID (eCommerce)
P06213

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.