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human ICT1 shRNA silencing AAV

Name 
AAV-h-ICT1-shRNA
Cat No 
shAAV-211760
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human MRPL58.

shAAV-211760
AAV-h-ICT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MRPL58 (mitochondrial ribosomal protein L58). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
39S ribosomal protein L58, mitochondrial; digestion substraction 1; DS1; DS-1; ICT1; immature colon carcinoma transcript 1 protein; mitochondrial large ribosomal subunit protein ICT1; mitochondrial large ribosomal subunit protein mL62; MRPL58; MRP-L58; peptidyl-tRNA hydrolase ICT1, mitochondrial
Description (eCommerce)
ICT1 is an essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q14197].
Description (Vector)
The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is ICT1 whose mRNA and protein were more highly expressed in undifferentiated than in differentiated cells.
Gene ID
3396
Gene Name (eCommerce)
mitochondrial ribosomal protein L58
Gene Name (Vector)
mitochondrial ribosomal protein L58
Gene Symbol
MRPL58
HGNC ID
HGNC:5359
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
621
Protein Name (eCommerce)
Peptidyl-tRNA hydrolase ICT1, mitochondrial
RefSeq ID
NM_001545
RefSeq Synonyms
NM_001545, NM_001303265, BC015335,
Research Areas
Cell Biology,Mitochondrion,Translational Control
Research Areas (Faceted)
cell_biology,transcription_translation
Species
human
Target Sentence
ICT1 is an essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q14197].
UniGene ID
Hs.407955
UniProt ID (eCommerce)
Q14197

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.