human HLCS shRNA silencing AAV

Name 
AAV-h-HLCS-shRNA
Cat No 
shAAV-211242
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human HLCS.

shAAV-211242
AAV-h-HLCS-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human HLCS (holocarboxylase synthetase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
biotin apo-protein ligase; biotin--acetyl-CoA-carboxylase ligase; biotin--methylcrotonoyl-CoA-carboxylase ligase; biotin--methylmalonyl-CoA-carboxytransferase ligase; biotin--protein ligase; HCS; Holocarboxylase synthetase; holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase); holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
Description (eCommerce)
Holocarboxylase synthase (HCS) is an enzyme that catalyzes the binding of biotin to carboxylases and histones. HCS plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism [taken from NCBI Entrez Gene (Gene ID: 3141)].
Description (Vector)
HLCS encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Gene ID
3141
Gene Name (eCommerce)
holocarboxylase synthetase
Gene Name (Vector)
holocarboxylase synthetase
Gene Symbol
HLCS
HGNC ID
HGNC:4976
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2181
ORF Size (bp)
2181 bp
Protein Name (eCommerce)
Biotin--protein ligase
RefSeq ID
NM_000411
RefSeq Synonyms
NM_001242785, NM_001242784, NM_000411, BC060787,
Research Areas
Histone Modification,Metabolism/Metabolic Process,Mitochondrion
Research Areas (Faceted)
cell_biology,metabolism
Species
human
Target Sentence
Holocarboxylase synthase (HCS) is an enzyme that catalyzes the binding of biotin to carboxylases and histones. HCS plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism [taken from NCBI Entrez Gene (Gene ID: 3141)].
UniGene ID
Hs.371350
UniProt ID (eCommerce)
P50747

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.