human HEXB shRNA silencing AAV

Name
AAV-h-HEXB-shRNA

Cat No
shAAV-210980

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human HEXB.

shAAV-210980

AAV-h-HEXB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human HEXB (hexosaminidase subunit beta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ENC-1AS; HEL-248; HEL-S-111

Description (Vector)

Hexosaminidase B is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II).

Gene ID

3074

Gene Name (Vector)

hexosaminidase subunit beta

Gene Symbol

HEXB

HGNC ID

HGNC:4879

ORF Size (aa)

1671

ORF Size (bp)

1671 bp

RefSeq ID

NM_000521

RefSeq Synonyms

NM_001292004, NM_000521, BC017378,

Species

human

UniGene ID

Hs.69293

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.