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human GCLC shRNA silencing AAV

Name 
AAV-h-GCLC-shRNA
Cat No 
shAAV-209745
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human GCLC.

shAAV-209745
AAV-h-GCLC-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GCLC (glutamate-cysteine ligase catalytic subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
GCL; GCS; GLCL; GLCLC
Description (Vector)
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
Gene ID
2729
Gene Name (Vector)
glutamate-cysteine ligase catalytic subunit
Gene Symbol
GCLC
HGNC ID
HGNC:4311
ORF Size (aa)
1914
ORF Size (bp)
1914 bp
RefSeq ID
NM_001498
RefSeq Synonyms
NM_001498, NM_001197115, BC039894,
Species
human
UniGene ID
Hs.654465

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.