human FBXO11 shRNA silencing AAV

Name
AAV-h-FBXO11-shRNA

Cat No
shAAV-208773

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human FBXO11.

shAAV-208773

AAV-h-FBXO11-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human FBXO11 (F-box protein 11). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

F-box only protein 11; FBX11; IDDFBA; PRMT9; protein arginine N-methyltransferase 9; ubiquitin protein ligase E3 component n-recognin 6; UBR6; UG063H01; VIT1; VIT-1; vitiligo-associated protein 1; vitiligo-associated protein VIT-1

Description (eCommerce)

FBXO11 is a member of the F-box protein family and component of the Skp1-Cullin1-F-box (SCF) ubiquitin ligase complex. FBXO11 has been shown to interact with p53 and suppress its activity. Suppression of p53 activity is achieved via Nedd8 conjugation to p53.

Description (Vector)

FBXO11 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. FBXO11 is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

Gene ID

80204

Gene Name (eCommerce)

F-box protein 11

Gene Name (Vector)

F-box protein 11

Gene Symbol

FBXO11

HGNC ID

HGNC:13590

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2532

ORF Size (bp)

2532 bp

Protein Name (eCommerce)

F-box only protein 11

RefSeq ID

NM_001190274

RefSeq Synonyms

NM_025133, NM_001190274, BC136480, BC130445,

Research Areas

Histone Modification,Neurobiology,p53 Signaling,Signal Transduction,Ubiquitination,Zinc-finger

Research Areas (Faceted)

cell_biology,neurobiology,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.352677

UniProt ID (eCommerce)

Q86XK2

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.