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human FAM105B shRNA silencing AAV

Name 
AAV-h-FAM105B-shRNA
Cat No 
shAAV-208296
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human OTULIN.

shAAV-208296
AAV-h-FAM105B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human OTULIN (OTU deubiquitinase with linear linkage specificity). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AIPDS; deubiquitinating enzyme otulin; FAM105B; family with sequence similarity 105, member B; GUM; OTU domain-containing deubiquitinase with linear linkage specificity; ubiquitin thioesterase Gumby; ubiquitin thioesterase otulin
Description (eCommerce)
Otulin is a deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96BN8].
Gene ID
90268
Gene Name (eCommerce)
OTU deubiquitinase with linear linkage specificity
Gene Name (Vector)
OTU deubiquitinase with linear linkage specificity
Gene Symbol
OTULIN
HGNC ID
HGNC:25118
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
897
ORF Size (bp)
897 bp
Protein Name (eCommerce)
Ubiquitin thioesterase otulin
RefSeq ID
NM_138348
RefSeq Synonyms
NM_138348, BC015392, BC007706,
Research Areas
Angiogenesis,Immunology,Innate Immunity,Signal Transduction,Wnt Signaling
Research Areas (Faceted)
cancer,developmental_biology,immunology,signal_transduction
Species
human
Target Sentence
Otulin is a deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96BN8].
UniGene ID
Hs.406335
UniProt ID (eCommerce)
Q96BN8

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.