human F8 shRNA silencing AAV

Name
AAV-h-F8-shRNA

Cat No
shAAV-208235

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human F8.

shAAV-208235

AAV-h-F8-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human F8 (coagulation factor VIII). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AHF; DXS1253E; F8B; F8C; FVIII; HEMA

Description (Vector)

F8 encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. F8 produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

Gene ID

2157

Gene Name (Vector)

coagulation factor VIII

Gene Symbol

HGNC ID

HGNC:3546

ORF Size (aa)

7056

ORF Size (bp)

7056 bp

RefSeq ID

NM_000132

RefSeq Synonyms

NM_019863, NM_000132, BC166700, BC022513,

Species

human

UniGene ID

Hs.654450

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.