human F10 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human F10.
shAAV-208215
AAV-h-F10-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human F10 (coagulation factor X). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
FX; FXA
Description (Vector)
F10 encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity.
Gene ID
2159
Gene Name (Vector)
coagulation factor X
Gene Symbol
F10
HGNC ID
HGNC:3528
ORF Size (aa)
1467
ORF Size (bp)
1467 bp
RefSeq ID
NM_000504
RefSeq Synonyms
NM_001312675, NM_001312674, NM_000504, BC046125,
Species
human
UniGene ID
Hs.361463
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.