human EWSR1 shRNA silencing AAV

Name 
AAV-h-EWSR1-shRNA
Cat No 
shAAV-208166
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human EWSR1.

shAAV-208166
AAV-h-EWSR1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human EWSR1 (EWS RNA binding protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
bK984G1.4; Ewing sarcoma breakpoint region 1; Ewing sarcoma breakpoint region 1 protein; Ewings sarcoma EWS-Fli1 (type 1) oncogene; EWS; EWS oncogene; EWS RNA-binding protein variant 6; EWS-FLI1; RNA-binding protein EWS
Description (eCommerce)
EWS is a member of a family of RNA-binding proteins that may serve as adapters that bridge RNA polymerase II complex and RNA splicing factors. Translocations and fusions between EWS and the ETS transcription factors promote oncogenesis and are associated with Ewing’s family tumors.
Description (Vector)
EWSR1 encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.
Gene ID
2130
Gene Name (eCommerce)
EWS RNA binding protein 1
Gene Name (Vector)
EWS RNA binding protein 1
Gene Symbol
EWSR1
HGNC ID
HGNC:3508
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1968
ORF Size (bp)
1968 bp
Protein Name (eCommerce)
RNA-binding protein EWS
RefSeq ID
NM_005243
RefSeq Synonyms
NM_013986, NM_005243, NM_001163287, NM_001163286, NM_001163285, BC072442, BC011048, BC004817, BC000527,
Research Areas
Cancer,RNA Binding,RNA Processing,Signal Transduction,TGF-beta Signaling,Transcription Factor/Regulator,Transcriptional Misregulation in Cancer,Tumor Suppressors/Oncoproteins,Zinc-finger
Research Areas (Faceted)
cancer,cell_biology,genetics,transcription_translation,immunology,signal_transduction
Species
human
Target Sentence
EWS is a member of a family of RNA-binding proteins that may serve as adapters that bridge RNA polymerase II complex and RNA splicing factors. Translocations and fusions between EWS and the ETS transcription factors promote oncogenesis and are associated with Ewing’s family tumors.
UniGene ID
Hs.374477
UniProt ID (eCommerce)
Q01844

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.