human EDN3 shRNA silencing AAV

Name
AAV-h-EDN3-shRNA

Cat No
shAAV-207568

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human EDN3.

shAAV-207568

AAV-h-EDN3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human EDN3 (endothelin 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ET3; HSCR4; MGC15067; MGC61498; WS4B;

Description (Vector)

EDN3 is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Four alternatively spliced transcript variants encoding three distinct isoforms have been observed.

Gene ID

1908

Gene Name (Vector)

endothelin 3

Gene Symbol

EDN3

HGNC ID

HGNC:3178

ORF Size (aa)

717

ORF Size (bp)

717 bp

RefSeq ID

NM_000114

RefSeq Synonyms

NM_207034, NM_207033, NM_207032, NM_001302456, NM_001302455, NM_000114, BC053866, BC008876,

Species

human

UniGene ID

Hs.1408

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.