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human DHCR7 shRNA silencing AAV

Name 
AAV-h-DHCR7-shRNA
Cat No 
shAAV-206932
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DHCR7.

shAAV-206932
AAV-h-DHCR7-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DHCR7 (7-dehydrocholesterol reductase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
7-dehydrocholesterol reductase; 7-DHC reductase; delta-7-dehydrocholesterol reductase; putative sterol reductase SR-2; SLOS; Sterol Delta(7)-reductase; sterol delta-7-reductase
Description (eCommerce)
7-dehydrocholesterol reductase (DHCR7) is an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol [taken from NCBI Entrez Gene (Gene ID: 1717)].
Description (Vector)
DHCR7 encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. DHCR7 is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.
Gene ID
1717
Gene Name (eCommerce)
7-dehydrocholesterol reductase
Gene Name (Vector)
7-dehydrocholesterol reductase
Gene Symbol
DHCR7
HGNC ID
HGNC:2860
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1428
ORF Size (bp)
1428 bp
Protein Name (eCommerce)
7-dehydrocholesterol reductase
RefSeq ID
NM_001360
RefSeq Synonyms
NM_001360, NM_001163817, BC000054,
Research Areas
Metabolism/Metabolic Process
Research Areas (Faceted)
cell_biology,metabolism
Species
human
Target Sentence
7-dehydrocholesterol reductase (DHCR7) is an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol [taken from NCBI Entrez Gene (Gene ID: 1717)].
UniGene ID
Hs.503134
UniProt ID (eCommerce)
Q9UBM7

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.