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human DGKG shRNA silencing AAV

Name 
AAV-h-DGKG-shRNA
Cat No 
shAAV-206924
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DGKG.

shAAV-206924
AAV-h-DGKG-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DGKG (diacylglycerol kinase gamma). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DAGK3; DGK-GAMMA
Description (Vector)
DGKG encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
1608
Gene Name (Vector)
diacylglycerol kinase gamma
Gene Symbol
DGKG
HGNC ID
HGNC:2853
ORF Size (aa)
2259
ORF Size (bp)
2259 bp
RefSeq ID
NM_001346
RefSeq Synonyms
NM_001346, NM_001080745, NM_001080744, BC112363, BC089411,
Species
human
UniGene ID
Hs.683449

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.