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human DGKB shRNA silencing AAV

Name 
AAV-h-DGKB-shRNA
Cat No 
shAAV-206922
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DGKB.

shAAV-206922
AAV-h-DGKB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DGKB (diacylglycerol kinase beta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DAGK2; DGK; DGK-BETA
Description (Vector)
Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene.
Gene ID
1607
Gene Name (Vector)
diacylglycerol kinase beta
Gene Symbol
DGKB
HGNC ID
HGNC:2850
ORF Size (aa)
2322
ORF Size (bp)
2322 bp
RefSeq ID
NM_004080
RefSeq Synonyms
NM_145695, NM_004080, BC143632, BC105005,
Species
human
UniGene ID
Hs.567255

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.