human DDIT3 shRNA silencing AAV

Name
AAV-h-DDIT3-shRNA

Cat No
shAAV-206706

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human DDIT3.

shAAV-206706

AAV-h-DDIT3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DDIT3 (DNA damage inducible transcript 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CEBPZ; CHOP; CHOP-10; CHOP10; GADD153

Description (Vector)

DDIT3 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Gene ID

1649

Gene Name (Vector)

DNA damage inducible transcript 3

Gene Symbol

DDIT3

HGNC ID

HGNC:2726

ORF Size (aa)

510

ORF Size (bp)

510 bp

RefSeq ID

NM_004083

RefSeq Synonyms

NM_004083, NM_001195057, NM_001195056, NM_001195055, NM_001195054, NM_001195053, BC003637,

Species

human

UniGene ID

Hs.690217

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.